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Research Paper

Development and Validation of Stability-indicating method for the determination of Pregabalin by RP-HPLC

Pregabalin is an anticonvulsant drug used for neuropathic pain. Pregabalin binds to the alpha-2-delta subunit of the voltage-gated calcium channel in the central nervous system. The aim of the present study was to develop the stability indicating, highly accurate precise and linear method for a related substance of the Pregabalin through the reverse phase HPLC and to validate as per the current ICH guideline. The optimize method uses a reverse phase column, Inertsil ODS 3V (250 mm × 4.6 mm, 5µ), mobile Phase of Di-ammonium hydrogen phosphate buffer (6.5 ± 0.05) and Acetonitrile through gradient flow rate of 0.8 ml/min. Keeping the column at temperature 25°C and using the sample amount 20 µL and detected all the impurities at 210 nm by the UV detector. In the developed method, elution of Pregabalin was at 11.5 min and all the eluted impurities were well separated and met the system suitability criteria. The precision is exemplified by a relative standard deviation of 1.4%. Method percentage mean recoveries of all the impurities are within the range (90.0% to 115.0%) as per the protocol. The method was found to be robust. Linearity coefficient for all Impurities was more than 0.999. The LOD obtained was 0.004% and LOQ is 0.012%. Stability-indicating forced degradation established studies to show results that there was no interference of any degraded products or external environment. A new highly accurate, precise and stability indicating method was developed for a related substance of Pregabalin with all the impurities well separate from the degradation product of Pregabalin.

Published by: Shweta More, Pratima Tatke

Author: Shweta More

Paper ID: V4I4-1205

Paper Status: published

Published: July 10, 2018

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Others

Present day education with reference to the present youth

Education is the essence of overall development. It is the basic need for living a satisfactory life. In the present scenario, a true education is the one which helps one to live a value based life with a touch of practical aspect. There has to be the development of head, heart, and hand for the overall development of a human being. The basic question is are we giving right education or just creating machines who deteriorate the meaning of a good human resource. Is the youth of the nation happy with the education system, why are we not able to have classrooms with creative thinking and innovative youths. A revolution is needed to bring out the best, which can be indeed done by overhauling the process of education which will produce game changers in the global market.

Published by: Runa Paul

Author: Runa Paul

Paper ID: V4I4-1188

Paper Status: published

Published: July 9, 2018

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Review Paper

Working width: The forgotten dimension

The primary concern of the clinician is the 3-dimensional cleansing of the root canal system. The length of the root canal is a dimension which has been studied since long. But advancements in the imaging techniques and growing research has shown that the apical third of the root canal system has varying shapes that can lead to difficulties in the efficient cleaning & complete elimination of bacteria and debris. This article attempts to review various aspects related to the apical third dimension, techniques & methods presently available for its gauging, factors affecting the measurement and instruments or systems which claim a 3-dimensional cleaning and shaping of the apical third.

Published by: Dr. Pradnya V. Bansode, Dr. M. B. Wavdhane, Dr. Seema D. Pathak, Dr. Hardik Rana, Dr. Vaishali U. Bhalerao

Author: Dr. Pradnya V. Bansode

Paper ID: V4I4-1189

Paper Status: published

Published: July 9, 2018

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Research Paper

Effect of microsystin toxicity on Nain (Cirrhinus mrigala H.)

Microcystis aeruginosa, cyanobacteria are frequent components of much fresh water marine ecosystem which often produce a hepatotoxin called microsytin. The toxin adversely affects the aquatic life forms, in the present study, its effect on Nain (Cirrhinus mrigala) was observed in biological parameters. In the fishes, the serum calcium after exposure to an acute concentration of microcystin for 96h, the values decreased significantly (P=0.05) to 9.9 mg 100 ml-1 compared to control (12.6 mg 100 ml-1). The acute exposure of microcystin also decreased the serum protein from 7.3 to 4.8. The fish showed hypercholesterolemia on exposure to acute and lethal concentrations of microcystin for 96h and both short (15-30 days) and long (45-90 days) term, respectively. The antioxidants, SOD activity did not change significantly in the liver, kidney or gills of fish that had been exposed to crushed cyanobacteria for 15 days at lethal concentration, however, the longer exposure (30 days) resulted in a significant increase (P=0.05) in the SOD activity in liver but induction in kidney and gills were not significant.

Published by: Madhumita Srivastava, Arun Kumar Srivastava

Author: Madhumita Srivastava

Paper ID: V4I4-1204

Paper Status: published

Published: July 9, 2018

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Research Paper

Authorization of data sharing in cloud

A Secure information sharing plan proposes by utilizing calculation called DSS-CP-ABE in light of Attribute-Based Encryption (ABE) technique to offer effective access control over ciphertext. To utilize intermediary servers for encryption and decoding tasks. In our approach, computational activities in ABE are directed on intermediary servers, which significantly lessen the computational overhead on customer side cell phones. Then, in DSS-CP-ABE, keeping in mind the end goal to keep up information protection, a variant ascribe is added to the entrance structure. The unscrambling key organization is changed with the goal that can be sent to the intermediary servers securely. Here present sluggish re-encryption and portrayal field of credits to decrease the repudiation overhead when managing the client disavowal issue. At last, we execute the information sharing model system in light of LDSS. The investigations demonstrate that DSS can enormously decrease the overhead on the customer side, which just presents an insignificant extra cost on the server side. Such an approach is useful to execute practical information sharing security plot on cell phones.

Published by: Tamilarasi S., Ramya V.

Author: Tamilarasi S.

Paper ID: V4I4-1198

Paper Status: published

Published: July 9, 2018

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Research Paper

In vitro propagation of pomegranate

An investigation on “Micropropagation of pomegranate (PunicagranatumL.) Var. Bhagwan” In the present investigation total five sub-experiments was carried out. In which CRD was applied for a laboratory experiment and RBD for field experiment to find out best explants, surface sterilants, shooting media, rooting media, and hardening mixtures. Regarding the suitability of explants, shoot tip was best for culture establishment. The study on surface sterilization revealed that explants treated with 0.1 percent mercuric chloride for 10 minutes showed the maximum survival percentage (90.58%) and less contamination percentage (9.42) in shoot tip explants. Shoot differentiation study showed that maximum number of shots per shoot tip explant (1.73) was recorded in treatment containing MS + BAP 2.0 mg/l, maximum shoot length (2.80 cm) was observed on medium containing MS + BAP 2.5 mg/l and maximum number of leaves (7.73) was recorded on medium containing MS +BAP 2.5mg/among the auxins used in the rooting experiment the maximum number of roots(9.66) per shoot tip explant was recorded in treatment containing ½ MS + NAA 6 mg/l. Maximum root length (3.20 cm) was found on medium containing ½ MS + IBA mg/l. While hardening in different hardening media, it was observed that maximum (71.87%) survival of plantlets from shoot tip explants was found on medium containing soil + sand (1:1v/v).

Published by: Gorad D. S., Shaikh S. A., Kallalbandi B. M., Mali A. S.

Author: Gorad D. S.

Paper ID: V4I4-1191

Paper Status: published

Published: July 9, 2018

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